What is ELISA p24?

The ELISA (enzyme-linked immunosorbent assay) is a widely used application for detecting and quantifying proteins and antigens from various samples. HIV p24 ELISA kits are available from several suppliers for investigations on infectious disease. The HIV p24 antigen is the protein that forms the virus’s capsid.

Can ELISA detect p24 antigen?

Most commercial HIV-1 p24 assays employ a standard ELISA format for the capture and detection of p24 antigen. These methods are capable of detecting in the range of 5–25 pg/ml of HIV-1 p24 antigen in the absence of signal amplification [4].

What is a p24 assay?

HIV-1 RNA, anti-HIV antibodies, and HIV-1 capsid protein (p24 antigen) are the main viral markers used to detect HIV infection, and to monitor disease progression. HIV-1 p24 tests are used in combination with anti-HIV antibody testing for early detection of HIV-1 infection [1, 2].

How long is p24 antigen detectable?

p24 (short for protein 24) is produced 2–3 weeks after infection and before antibodies are produced. p24 levels are only detectable for the next 1–2 months. However, by the time the p24 levels have dropped antibodies will be present.

When does p24 antigen become detectable?

When do p24 antigens appear?

P24 antigens are a part of the virus, and research indicates that they tend to appear within 2 weeks of HIV entering the body. A fourth-generation test can reliably detect HIV as soon as 1 month after a person contracts it.

What causes p24 antigen?

High levels of p24 are present in the blood serum of newly infected individuals during the short period between infection and seroconversion, making p24 antigen assays useful in diagnosing primary HIV infection.

What is the full meaning of p24?

The core protein of the human immunodeficiency virus (HIV). The presence of p24 antigen in the blood is a marker of uncontrolled HIV replication.

When does p24 antigen appear?

What is HIV-1 p24 ELISA kit?

This Human Immunodeficiency Virus type 1 (HIV-1) p24 / Capsid Protein p24 ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative measurement of HIV HIV Gag-p24 protein in Recombinant protein . It contains recombinant HIV HIV Gag-p24, and antibodies raised against the recombinant protein.

How much P24 is HIV-1 p24?

These methods are capable of detecting in the range of 5–25 pg/ml of HIV-1 p24 antigen in the absence of signal amplification [4]. At this level of sensitivity, it is difficult to consistently detect p24 antigen in HIV-positive individuals, especially in those cases where the infections are asymptomatic.

What are the calibration curves for the HIV-1 p24 antigen assay?

Calibration curves for determination of HIV-1 p24 antigen prepared for the conventional HM-ELISA (A) and the HM-ELISA+PSAM at different times of illumination (B). The calibration curve obtained after 8 min of illumination (B, closed large squares) provides the highest sensitivity of the assay, with a lower limit of detection of 0.2 pg/ml.

Why are LoDs for PE HIV-1 p24 antigen detection kits increasing?

Possibly due to variations in the commercially available PE HIV-1 p24 antigen detection kits, in the present study we achieved LODs approximately 2-fold higher than we reported previously.